Data di Pubblicazione:
2015
Citazione:
Mini invasive skeletal muscle biopsy technique with a tri-axial end cut needle / M. Invernizzi, M. Rizzi, S. Carda, C. Cisari, C.G. Molinari, F. Reno'. - In: EUROPEAN REVIEW FOR MEDICAL AND PHARMACOLOGICAL SCIENCES. - ISSN 1128-3602. - 19:13(2015 Jul), pp. 2446-2451.
Abstract:
OBJECTIVE: Skeletal Muscle Biopsy is a minor surgical procedure for the diagnosis of different neuromuscular pathological conditions and has recently gained popularity also in the research field of age-related muscular modifications and sarcopenia. Few studies focused on the application of mini-invasive muscular biopsy in both normal and pathological conditions.The aim of our study was to describe a mini invasive ultrasoundguided skeletal muscular biopsy technique in complete spinal cord injured (SCI) patients and healthy controls with a tri-axial end-cut needle. PATIENTS AND METHODS: Skeletal muscle biopsies were collected from 6 chronic SCI patients and 3 healthy controls vastus lateralis muscle with a tri-axial end cut needle Biopince -Angiotech. Muscle samples were stained for ATPase to determine fibers composition, moreover, gene expression of cyclooxygenase-1 (COX-1) and prostaglandin E2 receptor has been analyzed by Real Time RT-PCR. RESULTS: All the procedures were perfomed easily without failures and complications. Control tissue was macroscopically thicker than SCI one. Control specimen displayed an equal distribution of type I and type II fibers, while SCI sample displayed a prevalence of type II fibers SCI specimen displayed a significant reduction in COX-1 gene expression. This mini-invasive approach was easy, accurate and with low complication rate in performing skeletal muscle biopsy in both SCI patients and controls. CONCLUSIONS: This technique could be useful in conditions in which the overall quantity of specimen required is small like for molecular biology analysis. For histological diagnostic purposes and/or conditions in which the original tissue is already pathologically modified, this technique should be integrated with more invasive techniques.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
skeletal muscle; biopsy needle; image-guided biopsy; molecular biology
Elenco autori:
M. Invernizzi, M. Rizzi, S. Carda, C. Cisari, C.G. Molinari, F. Reno'
Link alla scheda completa: