Data di Pubblicazione:
2018
Citazione:
Photobiomodulation induces in vitro re-epithelialization via nitric oxide production / M. Rizzi, M. Migliario, S. Tonello, V. Rocchetti, F. Renò. - In: LASERS IN MEDICAL SCIENCE. - ISSN 0268-8921. - 33:5(2018 Jul), pp. 1003-1008. [10.1007/s10103-018-2443-7]
Abstract:
Photobiomodulation is a widely used tool in regenerative medicine thanks to its ability to modulate a plethora of physiological responses. Wound re-epithelialization is strictly regulated by locally produced chemical mediators, such as nitric oxide (NO), a highly reactive free radical generated by the nitric oxide synthase (NOS) enzymatic family. In this study, it has been hypothesized that a 980-nm low-level laser stimulation could increase NO production in human keratinocytes and that such event might be
directly related to the re-epithelialization process. Human keratinocytes were irradiated with increasing energy outputs (10–75 J) in the absence or presence of L-NAME, a NOS inhibitor. Laser stimulation induced an increase in NO production, resulting in an energy-dependent increase in both keratinocytes proliferation and re-epithelialization ability. The direct link between increased NO production and the observed physiological responses was confirmed by their inhibition in L-NAME pre-treated samples.
Since NO production increase is a quick event, it is conceivable that it is due to an increase in existing NOS activity rather than to a de novo protein synthesis. For this reason, it could be hypothesized that photobiomodulation-derived NO positive effects on keratinocytes behavior might rely on a near infrared mediated increase in NOS conformational stability and cofactors as well as substrate binding ability, finally resulting in an increased enzymatic activity.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
keratinocytes; low-level laser therapy; nitric oxide; re-epithelialization; surgery
Elenco autori:
M. Rizzi, M. Migliario, S. Tonello, V. Rocchetti, F. Renò
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