Cysteinyl-leukotrienes are released from astrocytes and increase astrocyte proliferation and glial fibrillary acidic protein via cys-LT1 receptors and mitogen-activated protein kinase pathway
Articolo
Data di Pubblicazione:
2004
Citazione:
Cysteinyl-leukotrienes are released from astrocytes and increase astrocyte proliferation and glial fibrillary acidic protein via cys-LT1 receptors and mitogen-activated protein kinase pathway / R. Ciccarelli, I. D'Alimonte, C. Santavenere, M. D'Auro, P. Ballerini, E. Nargi, S. Buccella, S. Nicosia, G. Folco, F. Caciagli, P. Di Iorio. - In: EUROPEAN JOURNAL OF NEUROSCIENCE. - ISSN 0953-816X. - 20:6(2004 Sep), pp. 1514-1524. [10.1111/j.1460-9568.2004.03613.x]
Abstract:
Cysteinyl-leukotrienes (cys-LTs), potent mediators in inflammatory diseases, are produced by nervous tissue, but their cellular source and role in the brain are not very well known. In this report we have demonstrated that rat cultured astrocytes express the enzymes (5'-lipoxygenase and LTC(4) synthase) required for cys-LT production, and release cys-LTs in resting condition and, to a greater extent, in response to calcium ionophore A23187, 1 h combined oxygen-glucose deprivation or 2-methyl-thioATP, a selective P2Y(1)/ATP receptor agonist. MK-886, a LT synthesis inhibitor, prevented basal and evoked cys-LT release. In addition, 2-methyl-thioATP-induced cys-LT release was abolished by suramin, a P2 receptor antagonist, or by inhibitors of ATP binding cassette proteins involved in cys-LT release. We also showed that astrocytes express cys-LT(1) and not cys-LT(2) receptors. The stimulation of these receptors by LTD(4) activated the mitogen-activated protein kinase (MAPK) pathway. This effect was: (i) insensitive to inhibitors of receptor-coupled Gi protein (pertussis toxin) or tyrosine kinase receptors (genistein); (ii) abolished by MK-571, a cys-LT(1) selective receptor antagonist, or PD98059, a MAPK inhibitor; (iii) reduced by inhibitors of calcium/calmodulin-dependent kinase II (KN-93), Ca(2+)-dependent and -independent (GF102903X) or Ca(2+)-dependent (Gö6976) protein kinase C isoforms. LTD(4) also increased astrocyte proliferation and glial fibrillary acidic protein content, which are considered hallmarks of reactive astrogliosis. Both effects were counteracted by cell pretreatment with MK-571 or PD98059. Thus, cys-LTs released from astrocytes might play an autocrine role in the induction of reactive astrogliosis that, in brain injuries, contributes to the formation of a reparative glial scar.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Astrogliosis; cys-LT1 receptors; Cysteinyl-leukotriene release; MAPK pathway; Rat astrocytes
Elenco autori:
R. Ciccarelli, I. D'Alimonte, C. Santavenere, M. D'Auro, P. Ballerini, E. Nargi, S. Buccella, S. Nicosia, G. Folco, F. Caciagli, P. Di Iorio
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