Archival skin biopsy specimens as a tool for miRNA-based diagnosis: Technical and post-analytical considerations
Articolo
Data di Pubblicazione:
2023
Citazione:
Archival skin biopsy specimens as a tool for miRNA-based diagnosis: Technical and post-analytical considerations / M. Andelic, M. Marchi, S. Marcuzzo, R. Lombardi, C.G. Faber, G. Lauria, E. Salvi. - In: MOLECULAR THERAPY. METHODS & CLINICAL DEVELOPMENT. - ISSN 2329-0501. - (2023), pp. 101116.1-101116.33. [Epub ahead of print] [10.1016/j.omtm.2023.101116]
Abstract:
Archived specimens, taken by standardized procedures in clinical practice, represent a valuable resource in translational medicine. Their use in retrospective molecular-based studies could provide disease and therapy predictors. Microfluidic array is user-friendly and cost-effective method allowing profiling of hundreds of microRNAs from low amount of RNA. However, even though tissue microRNAs may include potentially robust biomarkers, non-uniformed post-analytical pipelines could hinder translation into clinics. In this study, epidermal RNA from archival skin biopsy specimens was isolated from patients with peripheral neuropathy and healthy individuals. Unbiased miRNA profiling was performed using RT-qPCR-based microfluidic array. We demonstrated that RNA obtained from archival tissue is appropriate for miRNA profiling, providing evidence that different practices in threshold selection could significantly influence the final results. We showed the utility of software-based quality control for amplification curves. We revealed that selection of the most stable reference and the calculation of geometric mean are suitable when utilizing microfluidic arrays without known references. By applying appropriate post-analytical settings, we obtained miRNA profile of human epidermis associated with biological processes and a list of suitable references. Our results, which outline technical and post-analytical considerations, support the broad use of archived specimens for miRNA analysis to unravel disease-specific molecular signatures.
Tipologia IRIS:
01 - Articolo su periodico
Elenco autori:
M. Andelic, M. Marchi, S. Marcuzzo, R. Lombardi, C.G. Faber, G. Lauria, E. Salvi
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