Data di Pubblicazione:
2010
Citazione:
Identification of the folding inhibitors of hen-egg lysozyme : gathering the right tools / M. Caldarini, L. Sutto, C. Camilloni, F. Vasile, R. A. Broglia, G. Tiana. - In: EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS. - ISSN 0175-7571. - 39:6(2010), pp. 911-919.
Abstract:
The unfolded state of proteins displays a surprisingly rich amount of local native structure, which appears to be critical for driving the protein to its native state. Peptides with the same sequence of the corresponding structured segments can be used to interfere with the correct folding of the protein. Using model simulations, we investigate the folding of hen-egg lysozyme, identifying its key segments. Activity assays, NMR and circular dichroism experiments are used to screen the peptides which are able to inhibit the folding of lysozyme. Few peptides, corresponding to the segments of the protein which are structured in the unfolded state, are identified to have significant inhibitory effects.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Folding inhibition
Elenco autori:
M. Caldarini, L. Sutto, C. Camilloni, F. Vasile, R. A. Broglia, G. Tiana
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