Data di Pubblicazione:
2010
Citazione:
Grapevine miRNAs : Towards A Structural And Functional Characterization / E. Mica, E. Bertolini, V. Piccolo, D. Lacourieux, D. Horner, E. Pè. ((Intervento presentato al 18. convegno Plant and Animal Genomes tenutosi a San Diego, CA nel 2010.
Abstract:
In plants, microRNAs (miRNAs) are key post-transcriptional regulatory elements approximately 21 nt long, coded by MIRNA genes, which regulate
plant architecture, nutrient homeostasis and stress response. The crucial role they play in fine tuning gene regulation clearly implies that a
complete characterization of genomes structure and function cannot be attained without a deep analysis of this class of regulatory elements. Here
we present the characterization of miRNA genes in grapevine, following the annotation of 140 conserved MIRNA genes (Jaillon et al., 2007).
Starting from the description of their transcriptional landscape (Mica et al., 2009) we analyzed their genomic structure and interaction with putative
targets. We experimentally validated primary transcript boundaries and alternative splicing events of several miRNA genes, confirming bioinformatic
predictions and showing different splicing patterns and alternative transcription end points. Putative targets were identified and validated by means
of 5’ modified RACE experiments. We are currently focusing on target genes involved in several physiological pathways such as phenylpropanoid
pathway and that, according to transcriptome analysis using oligo-chips, are up- or down-regulated in berries after light and heat stress
treatments.
Tipologia IRIS:
14 - Intervento a convegno non pubblicato
Keywords:
Grapevine, miRNAs
Elenco autori:
E. Mica, E. Bertolini, V. Piccolo, D. Lacourieux, D. Horner, E. Pè
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