Mutations in the external loops of BK virus VP1 and urine viral load in renal transplant recipients
Articolo
Data di Pubblicazione:
2010
Citazione:
Mutations in the external loops of BK virus VP1 and urine viral load in renal
transplant recipients / S. Tremolada, S. Delbue, L. Castagnoli, S. Allegrini, U. Miglio, R. Boldorini, F. Elia,
J. Gordon, P. Ferrante. - In: JOURNAL OF CELLULAR PHYSIOLOGY. - ISSN 0021-9541. - 222:1(2010), pp. 195-199.
Abstract:
Polyomavirus-associated nephropathy (PVAN) is a major complication that occurs
after renal transplantation and is induced by reactivation of the human
polyomavirus BK (BKV). The structure of the viral capsid protein 1 (VP1) is
characterized by the presence of external loops, BC, DE, EF, GH, and HI, which
are involved in receptor binding. The pathogenesis of PVAN is not well
understood, but viral risk factors are thought to play a crucial role in the
onset of this pathology. In an attempt to better understand PVAN pathogenesis,
the BKV-VP1 coding region was amplified, cloned, and sequenced from the urine of
kidney transplant recipients who did, and did not, develop the pathology. Urine
viral loads were determined by using real time quantitative PCR (Q-PCR). Amino
acid substitutions were detected in 6/8 patients, and 6/7 controls. The BC and EF
loop regions were most frequently affected by mutations, while no mutations were
found within the GH and HI loops of both patients and controls. Some mutations,
that were exclusively detected in the urine of PVAN patients, overlapped with
previously reported mutations, although a correlation between changes in amino
acids and the development of PVAN was not found. Urine viral loads were higher
than that of the proposed cut-off loads for identification of patients that are
at a high risk of developing PVAN (10(7) copies/ml), both in the PVAN and control
groups, thus confirming that urine viral load is not a useful predictive marker
for the development of PVAN
Tipologia IRIS:
01 - Articolo su periodico
Elenco autori:
S. Tremolada, S. Delbue, L. Castagnoli, S. Allegrini, U. Miglio, R. Boldorini, F. Elia,
J. Gordon, P. Ferrante
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