Detection of human papillomavirus in fresh and dried urine through an automated system for cervical cancer screening in low‐ and middle‐income countries
Articolo
Data di Pubblicazione:
2023
Citazione:
Detection of human papillomavirus in fresh and dried urine through an automated system for cervical cancer screening in low‐ and middle‐income countries / E. Tanzi, S. Bianchi, C. Fappani, M. Gori, D. Colzani, I. Passera, C. Tincati, M. Canuti, M.C.B. Raviglione, A. Amendola. - In: JOURNAL OF MEDICAL VIROLOGY. - ISSN 0146-6615. - 95:5(2023 May), pp. e28802.1-e28802.9. [10.1002/jmv.28802]
Abstract:
The majority of cervical cancer cases and associated deaths occur in low‐ and
middle‐income countries (LMICs), where sociocultural barriers, poor access to
prevention and care, and technical and practical difficulties hinder screening
coverage improvement. Using urine specimens for human papillomaviruses (HPV)
molecular screening through automated testing platforms can help to overcome
these problems. We evaluated the high‐risk (HR) HPV detection performance of
the Xpert ® HPV test on GeneXpert ® System (Cepheid), on fresh and dried urine
(Dried Urine Spot [DUS]) samples as compared to an in‐house polymerase chain
reaction (PCR) genotyping assay. Forty‐five concentrated urine samples collected
from women with known cytological and HPV infection status, determined through
in‐house PCR and genotyping assays, were tested “as is” and as DUS with the Xpert®
HPV test. This system detected HR‐HPV in 86.4% of fresh and in 77.3% of dried
urine samples collected from HPV+ women, correctly identifying HR‐HPV infection
in 100% of women with low‐ and high‐grade lesions. High concordance (91.4%,
k = 0.82) was found between PCR test and Xpert ® HPV Test from urine. Urine‐based
Xpert ® HPV test seems to be a suitable screening test for detection of HR‐HPV
infections associated with low‐ and high‐grade lesions requiring follow‐up
monitoring or treatment. This methodology, relying on noninvasively collected
samples and on available rapid testing platforms, could facilitate large, at‐scale
screening programs, particularly in LMICs and rural areas, thus reducing adverse
outcomes of HPV infection and facilitating achievement of the WHO cervical cancer
elimination goal.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Dried Urine Spot; HPV; low- and middle-income countries; molecular screening; urine
Elenco autori:
E. Tanzi, S. Bianchi, C. Fappani, M. Gori, D. Colzani, I. Passera, C. Tincati, M. Canuti, M.C.B. Raviglione, A. Amendola
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