Data di Pubblicazione:
2023
Citazione:
Biomolecular Monitoring Tool Based on Lab-on-Chip for Virus Detection / F. Costantini, N. Lovecchio, M. Nandimandalam, A. Manglli, F. Faggioli, M. Biasin, C. Manetti, P.F. Roversi, A. Nascetti, G. de Cesare, D. Caputo. - In: BIOSENSORS. - ISSN 2079-6374. - 13:5(2023), pp. 544.1-544.14. [10.3390/bios13050544]
Abstract:
: Lab-on-Chip (LoC) devices for performing real-time PCR are advantageous compared to
standard equipment since these systems allow to conduct in-field quick analysis. The development
of LoCs, where the components for performing the nucleic acid amplification are all integrated, can
be an issue. In this work, we present a LoC-PCR device where thermalization, temperature control
and detection elements are all integrated on a single glass substrate named System-on-Glass (SoG)
obtained using metal thin-film deposition. By using a microwell plate optically coupled with the
SoG, real-time reverse transcriptase PCR of RNA extracted from both a plant and human virus has
been carried out in the developed LoC-PCR device. The limit of detection and time of analysis
for the detection of the two viruses by using the LoC-PCR were compared with those achieved by
standard equipment. The results showed that the two systems can detect the same concentration
of RNA; however, the LoC-PCR performs the analysis in half of the time compared to the standard
thermocycler, with the advantage of the portability, leading to a point-of-care device for several
diagnostic applications.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Lab-on-Chip; real-time RT-PCR; System-on-Glass; virus detection
Elenco autori:
F. Costantini, N. Lovecchio, M. Nandimandalam, A. Manglli, F. Faggioli, M. Biasin, C. Manetti, P.F. Roversi, A. Nascetti, G. de Cesare, D. Caputo
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