Toxicological Profile of PM from Different Sources in the Bronchial Epithelial Cell Line BEAS-2B
Articolo
Data di Pubblicazione:
2023
Citazione:
Toxicological Profile of PM from Different Sources in the Bronchial Epithelial Cell Line BEAS-2B / G. Melzi, E. Nozza, M.A. Frezzini, S. Canepari, R. Vecchi, L. Cremonesi, M. Potenza, M. Marinovich, E. Corsini. - In: TOXICS. - ISSN 2305-6304. - 11:5(2023), pp. 413.1-413.13. [10.3390/toxics11050413]
Abstract:
The toxicity of particulate matter (PM) is strictly associated with its physical-chemical
characteristics, such as size or chemical composition. While these properties depend on the origin
of the particles, the study of the toxicological profile of PM from single sources has rarely been
highlighted. Hence, the focus of this research was to investigate the biological effects of PM from five
relevant sources of atmospheric PM: diesel exhaust particles, coke dust, pellet ashes, incinerator ashes,
and brake dust. Cytotoxicity, genotoxicity, oxidative, and inflammatory response were assessed
in a bronchial cell line (BEAS-2B). BEAS-2B cells were exposed to different concentrations (25, 50,
100, and 150 μg/mL medium) of particles suspended in water. The exposure lasted 24 h for all
the assays performed, except for reactive oxygen species, which were evaluated after 30 min, 1 h,
and 4 h of treatment. The results showed a different action of the five types of PM. All the tested
samples showed a genotoxic action on BEAS-2B, even in the absence of oxidative stress induction.
Pellet ashes seemed to be the only ones able to induce oxidative stress by boosting the formation of
reactive oxygen species, while brake dust resulted in the most cytotoxic. In conclusion, the study
elucidated the differential response of bronchial cells to PM samples generated by different sources.
The comparison could be a starting point for a regulatory intervention since it highlighted the toxic
potential of each type of PM tested.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
diesel exhaust particle; coke dust; incinerator ash; pellet ash; brake dust; genotoxicity; oxidative stress; BEAS-2B; in vitro
Elenco autori:
G. Melzi, E. Nozza, M.A. Frezzini, S. Canepari, R. Vecchi, L. Cremonesi, M. Potenza, M. Marinovich, E. Corsini
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