The integration of mutant loci affecting maize endosperm development in a dense genetic map using an AFLP-based procedure
Articolo
Data di Pubblicazione:
2008
Citazione:
The integration of mutant loci affecting maize endosperm development in a dense genetic map using an AFLP-based procedure / L. Pasini, M.R. Stile, E. Puja, R. Valsecchi, P. Francia, G. Carletti, F. Salamini, A. Marocco. - In: MOLECULAR BREEDING. - ISSN 1380-3743. - 22:4(2008), pp. 527-541.
Abstract:
In this paper, 10 mutations conditioning
the appearance of defective, miniature or collapsed endosperm, but with normal sporophyte development, were considered. Homozygous mutant kernels have reduced grain weight, kernel size, density and, in some of these, higher than normal seed protein content. The mutant loci were integrated into a highresolution
genetic map in order to associate them to
specific genes. We have placed 1167 AFLP markers
on a consensus map using IBM2 as a backbone and
reaching an average of 1 marker every 1.9 cM. We
have identified AFLP markers linked to all individual
mutant alleles. BSA was adopted to screen the largest
possible number of primer combinations on homozygous
F3 mutant and wild type plants. The ten mutant loci are linked to the closest AFLP or SSR
markers with distances ranging from 0 to 17.9 cM.
The genes we have defined by the existence of
mendelian mutants can now be considered good
candidates for testing the association to QT loci.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Bulked segregant analysis; Integrated molecular maps; Viable endosperm mutants; Zea mays
Elenco autori:
L. Pasini, M.R. Stile, E. Puja, R. Valsecchi, P. Francia, G. Carletti, F. Salamini, A. Marocco
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