An international validation study of the interleukin-2 luciferase leukocyte toxicity test (IL-2 Luc LTT) to evaluate potential immunosuppressive chemicals and its performance after use with the interleukin-2 luciferase assay (IL-2 Luc assay)
Articolo
Data di Pubblicazione:
2023
Citazione:
An international validation study of the interleukin-2 luciferase leukocyte toxicity test (IL-2 Luc LTT) to evaluate potential immunosuppressive chemicals and its performance after use with the interleukin-2 luciferase assay (IL-2 Luc assay) / K. Yutaka, Y. Rie, T. Iwaki, C. Fujimura, Y. Ohmiya, Y. Nakajima, T. Omori, E. Corsini, T. Inoue, E.L. Rogen, H. Kojima, S. Aiba. - In: TOXICOLOGY IN VITRO. - ISSN 0887-2333. - 88:(2023 Apr), pp. 105535.1-105535.8. [10.1016/j.tiv.2022.105535]
Abstract:
We previously reported that the IL-2 Luc LTT can detect immunosuppressive effects of drugs that are attributed to their antimitotic activity. Here, we report an official validation study of the IL-2 Luc LTT. In the Phase I study that evaluated five coded chemicals, the within-laboratory reproducibility of three independent laboratories was 100.0%. In the combined results of the Phase I and II studies that evaluated 20 coded chemicals, the betweenlaboratory reproducibility was 92.0%. When compared with the reference data based on the previously-reported immunotoxicological information, the predictivity of the combined Phase I and II studies was 76.0% for Lab A and 72.0% for Labs B and C. In contrast, in the study in which the lead laboratory examined 37 nonpharmaceutical chemicals, the predictivity of the IL-2 Luc LTT and the IL-2 Luc assay was 48.6% and 64.9%, respectively, whereas that of the combined assays was 74.3%. It is clear that an integrated approach combining multiple assays is necessary for the development of in vitro immunosuppression testing. These data suggest that the IL-2 Luc LTT alone is not sufficient as a component of the integrated approach, but the combination of the IL2 Luc assay and IL-2 Luc LTT is promising.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Antimitotic activity; IATA; IL-2; Immunotoxicity test
Elenco autori:
K. Yutaka, Y. Rie, T. Iwaki, C. Fujimura, Y. Ohmiya, Y. Nakajima, T. Omori, E. Corsini, T. Inoue, E.L. Rogen, H. Kojima, S. Aiba
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