Investigating the Impact of Delivery System Design on the Efficacy of Self-Amplifying {RNA} Vaccines
Articolo
Data di Pubblicazione:
2020
Citazione:
Investigating the Impact of Delivery System Design on the Efficacy of Self-Amplifying {RNA} Vaccines / G. Anderluzzi, G. Lou, S. Gallorini, M. Brazzoli, R. Johnson, D.T. O'Hagan, B.C. Baudner, Y. Perrie. - In: VACCINES. - ISSN 2076-393X. - 8:2(2020 Jun), pp. 212.1-212.22. [10.3390/vaccines8020212]
Abstract:
messenger RNA (mRNA)-based vaccines combine the positive attributes of both live-attenuated and subunit vaccines. In order for these to be applied for clinical use, they require to be formulated with delivery systems. However, there are limited in vivo studies which compare different delivery platforms. Therefore, we have compared four different cationic platforms: (1) liposomes, (2) solid lipid nanoparticles (SLNs), (3) polymeric nanoparticles (NPs) and (4) emulsions, to deliver a self-amplifying mRNA (SAM) vaccine. All formulations contained either the non-ionizable cationic lipid 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP) or dimethyldioctadecylammonium bromide (DDA) and they were characterized in terms of physico-chemical attributes,in vitro transfection efficiency and in vivo vaccine potency. Our results showed that SAM encapsulating DOTAP polymeric nanoparticles, DOTAP liposomes and DDA liposomes induced the highest antigen expression in vitro and, from these, DOTAP polymeric nanoparticles were the most potent in triggering humoral and cellular immunity among candidates in vivo.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
antigen expression; emulsions; immunogenicity; liposomes; polymeric nanoparticles; self-amplifying RNA; solid lipid nanoparticles
Elenco autori:
G. Anderluzzi, G. Lou, S. Gallorini, M. Brazzoli, R. Johnson, D.T. O'Hagan, B.C. Baudner, Y. Perrie
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