A Self-Assembled Non-Viral vector as Potential Platform for mRNA-Based Vaccines

Here, we show a universal anti-cancer vaccine, based on antigen-mRNA-loaded
self-assembled polyplex nanocarrier. The establishment of antigen-specific T-cells,
as consequence of the vaccination, performed following a subcutaneous route of
administration, was confirmed by detection of IFN-γ/IL-2 producing T-cells in the
spleen of the treated mice. Moreover, a high release of Th1-releated IgG isotypes
(IgG2b and IgG2c) was observed, indicating a predominantly Th1 response. Finally,
OVA-mRNA-based vaccine formulation has been employed for the treatment
of melanoma lung metastasis of B16-OVA challenged mice, inducing a marked
reduction of metastatic nodules up to 93%.
Ascertained that any polypeptide-based antigen can be encoded as RNA, potentially
our platform can represent a universal strategy suitable for the development of
any mRNA-based vaccine.