Cluster-Assembled Zirconia Substrates Accelerate the Osteogenic Differentiation of Bone Marrow Mesenchymal Stem Cells
Articolo
Data di Pubblicazione:
2023
Citazione:
Cluster-Assembled Zirconia Substrates Accelerate the Osteogenic Differentiation of Bone Marrow Mesenchymal Stem Cells / S. Castiglioni, L. Locatelli, A. Cazzaniga, F.M. Orecchio, T. Santaniello, C. Piazzoni, L. Bureau, F. Borghi, P. Milani, J.A. Maier. - In: NANOMATERIALS. - ISSN 2079-4991. - 13:5(2023 Feb 22), pp. 801.1-801.14. [10.3390/nano13050801]
Abstract:
Due to their high mechanical strength and good biocompatibility, nanostructured zirconia
surfaces (ns-ZrOx) are widely used for bio-applications. Through supersonic cluster beam deposition,
we produced ZrOx films with controllable roughness at the nanoscale, mimicking the morphological
and topographical properties of the extracellular matrix. We show that a 20 nm ns-ZrOx surface ac-
celerates the osteogenic differentiation of human bone marrow-derived MSCs (bMSCs) by increasing
the deposition of calcium in the extracellular matrix and upregulating some osteogenic differentiation
markers. bMSCs seeded on 20 nm ns-ZrOx show randomly oriented actin fibers, changes in nuclear
morphology, and a reduction in mitochondrial transmembrane potential when compared to the cells
cultured on flat zirconia (flat-ZrO2) substrates and glass coverslips used as controls. Additionally,
an increase in ROS, known to promote osteogenesis, was detected after 24 h of culture on 20 nm
ns-ZrOx. All the modifications induced by the ns-ZrOx surface are rescued after the first hours of
culture. We propose that ns-ZrOx-induced cytoskeletal remodeling transmits signals generated by
the extracellular environment to the nucleus, with the consequent modulation of the expression of
genes controlling cell fate.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
nanostructured zirconia surfaces; bMSC; osteogenic differentiation
Elenco autori:
S. Castiglioni, L. Locatelli, A. Cazzaniga, F.M. Orecchio, T. Santaniello, C. Piazzoni, L. Bureau, F. Borghi, P. Milani, J.A. Maier
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