Characterization and analysis of the expression pattern of microRNAs in the grapevine Vitis vinifera
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Data di Pubblicazione:
2008
Citazione:
Characterization and analysis of the expression pattern of microRNAs in the grapevine Vitis vinifera / V. Piccolo, E. Mica, E. Pè, G. Pesole, D. Horner. ((Intervento presentato al 10. convegno FISV Annual Congress tenutosi a Riva del Garda nel 2008.
Abstract:
MicroRNAs are small (19-24 nt) noncoding RNAs that play an important role in the regulation of multiple
cell events, inhibiting gene expression at the posttranscriptional level by binding target mRNAs that are
subsequently degraded or sequestered from translation. The availability of the complete genome
sequence of the grapevine (Vitis vinifera), has already permitted genome-wide predictions of microRNAs
by purely computational methods. Here we integrate transcriptomic data derived from high-throughput
Illumina SOLEXA and ABI SOLiD sequence tags derived from both polyA+ transcripts and isolated small
RNAs with oligonucleotide array data. We are thus able to detect both mature microRNAs and to
establish whether genomic loci corresponding to the pre-miRNA are expressed in various tissues. In
many cases, the unambiguous alignment of sequence tags derived from polyA+ RNA to the genomic
sequence allow provisional mapping of primary microRNA transcripts, a first step towards bioinformatics
characterization of elements potentially regulating microRNA expression.
Tipologia IRIS:
14 - Intervento a convegno non pubblicato
Elenco autori:
V. Piccolo, E. Mica, E. Pè, G. Pesole, D. Horner
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