Whole-Genome Sequencing of hMPXV1 in five Italian cases confirms the occurrence of the predominant epidemic lineage
Articolo
Data di Pubblicazione:
2023
Citazione:
Whole-Genome Sequencing of hMPXV1 in five Italian cases confirms the occurrence of the predominant epidemic lineage / D. Forni, C. Moltrasio, M. Sironi, A. Mozzi, E. Quattri, L. Venegoni, M. Zamprogno, A. Citterio, M. Clerici, A.V. Marzano, R. Cagliani. - In: JOURNAL OF MEDICAL VIROLOGY. - ISSN 1096-9071. - 95:2(2023), pp. e28493.1-e28493.7. [10.1002/jmv.28493]
Abstract:
The ongoing outbreak of monkeypox virus (hMPXV1) is the largest recorded in historically non-endemic countries. Genomic surveillance has emerged as a pivotal tool to track the spread and monitor the evolution of viral pathogens. Therefore, to assess the genetic diversity of circulating hMPXV1 in northern Italy in June-July 2022, we sequenced and analyzed five complete genomes of viruses sampled from patients presenting with a typical course of hMPXV1 infection. Phylogenetic analysis confirmed that all five genomes belong to the predominant epidemic lineage (B.1). Inspection of genetic changes and comparison with the reference sequence showed the presence of twelve nucleotide substitutions. Seven are non-synonymous mutations leading to amino acid changes in six proteins belonging to different functional classes. Moreover, eleven of these twelve nucleotide mutations involve GA>AA or TC >TT replacements, suggesting that host APOBEC3 enzymes are responsible for the generation of substitutions in circulating viruses. Finally, metagenomic analysis evidenced bacterial superinfection (Streptococcus pyogenes) in one patient. Through this study, we contributed to expand the number of complete genomes of viruses circulating in Italy and characterize them as belonging to the predominant outbreak lineage.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Monkeypox; Whole-Genome Sequencing; genomic surveillance; hMPXV1
Elenco autori:
D. Forni, C. Moltrasio, M. Sironi, A. Mozzi, E. Quattri, L. Venegoni, M. Zamprogno, A. Citterio, M. Clerici, A.V. Marzano, R. Cagliani
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