A novel promising laccase from the psychrotolerant and halotolerant Antarctic marine Halomonas sp. M68 strain
Articolo
Data di Pubblicazione:
2023
Citazione:
A novel promising laccase from the psychrotolerant and halotolerant Antarctic marine Halomonas sp. M68 strain / M. Bisaccia, E. Binda, E. Rosini, G. Caruso, O. Dell'Acqua, M. Azzaro, P. Laganà, G. Tedeschi, E.M. Maffioli, L. Pollegioni, F. Marinelli. - In: FRONTIERS IN MICROBIOLOGY. - ISSN 1664-302X. - 14:(2023 Feb 10), pp. 1-13. [10.3389/fmicb.2023.1078382]
Abstract:
Microbial communities inhabiting the Antarctic Ocean show psychrophilic and
halophilic adaptations conferring interesting properties to the enzymes they produce,
which could be exploited in biotechnology and bioremediation processes. Use of
cold- and salt-tolerant enzymes allows to limit costs, reduce contaminations, and
minimize pretreatment steps. Here, we report on the screening of 186 morphologically
diverse microorganisms isolated from marine biofilms and water samples collected
in Terra Nova Bay (Ross Sea, Antarctica) for the identification of new laccase activities.
After primary screening, 13.4 and 10.8% of the isolates were identified for the ability
to oxidize 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and the dye
azure B, respectively. Amongst them, the marine Halomonas sp. strain M68 showed
the highest activity. Production of its laccase-like activity increased six-fold when
copper was added to culture medium. Enzymatic activity-guided separation coupled
with mass spectrometry identified this intracellular laccase-like protein (named Ant
laccase) as belonging to the copper resistance system multicopper oxidase family.
Ant laccase oxidized ABTS and 2,6-dimethoxy phenol, working better at acidic pHs
The enzyme showed a good thermostability, with optimal temperature in the 40–
50°C range and maintaining more than 40% of its maximal activity even at 10°C.
Furthermore, Ant laccase was salt- and organic solvent-tolerant, paving the way
for its use in harsh conditions. To our knowledge, this is the first report concerning
the characterization of a thermo- and halo-tolerant laccase isolated from a marine
Antarctic bacterium.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Antarctica; laccase; Halomonas sp.; marine biofilm; cold-adaptation; extremophile bacteria
Elenco autori:
M. Bisaccia, E. Binda, E. Rosini, G. Caruso, O. Dell'Acqua, M. Azzaro, P. Laganà, G. Tedeschi, E.M. Maffioli, L. Pollegioni, F. Marinelli
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