Experimental evidence on the efficacy of two new metabolic modulators on mitochondrial biogenesis and function in mouse cardiomyocytes
Articolo
Data di Pubblicazione:
2020
Citazione:
Experimental evidence on the efficacy of two new metabolic modulators on mitochondrial biogenesis and function in mouse cardiomyocytes / L. Tedesco, F. Rossi, C. Ruocco, M. Ragni, M.O. Carruba, A. Valerio, E. Nisoli. - In: CANADIAN JOURNAL OF CLINICAL PHARMACOLOGY. - ISSN 1710-6222. - 27:special issue 2(2020 Sep 16), pp. e12-e21. [10.15586/jptcp.v27iSP2.740]
Abstract:
Proper maintenance of mitochondria' homeostasis is essential for cell health, and mitochondrial dysfunction underlies several metabolic and heart diseases. Stimulation of mitochondrial biogenesis represents a valuable therapeutic tool for the prevention and treatment of disorders characterized by a deficit in energy metabolism. The present study aimed to potentiate the mitochondria' biogenetic efficacy of an amino acid (AA) mixture, enriched in branched-chain amino acids (BCAAs), Which we previously showed to boost mitochondrial biogenesis, leading to life span extension and reducing of muscle and liver damage. Hence, we designed and studied several innovative mixtures. Here, we report on two new AA formulas, a5 and E7, created on the BCAA-enriched amino acid mixture (BCAAem) template and enriched with Krebs cycle substrates, including succinatc, malatc, and citrate. Cardiomyocytes in culture exposed to either mixture showed increased mitochondrial DNA amount, mitochondrial biogenesis markers, and oxygen consumption. Furthermore, a5 and E7 also increased the expression of BCAA catabolic genes. Most importantly, all of these effects of a5 and E7 were more pronounced than those observed with BCAAem, confirming the higher mitochondrial biogenesis potential of these new formulas. Therefore, a5 and E7 could represent a more efficient tool for the nutritional treatment of diseases in which energy production is defective.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
branched-chain amino acids; cardiomyocytes; essential amino acids; mitochondria; tricaboxylic acid cycle
Elenco autori:
L. Tedesco, F. Rossi, C. Ruocco, M. Ragni, M.O. Carruba, A. Valerio, E. Nisoli
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