Targeting a Multidrug-Resistant Pathogen: First Generation Antagonists of Burkholderia cenocepacia's BC2L-C Lectin
Articolo
Data di Pubblicazione:
2022
Citazione:
Targeting a Multidrug-Resistant Pathogen: First Generation Antagonists of Burkholderia cenocepacia's BC2L-C Lectin / R. Bermeo, K. Lal, D. Ruggeri, D. Lanaro, S. Mazzotta, F. Vasile, A. Imberty, L. Belvisi, A. Varrot, A. Bernardi. - In: ACS CHEMICAL BIOLOGY. - ISSN 1554-8929. - 17:10(2022 Oct 21), pp. 2899-2910. [10.1021/acschembio.2c00532]
Abstract:
Multidrug-resistant pathogens such as Burkholderia cenocepacia have become a hazard in the context of healthcare-associated infections, especially for patients admitted with cystic fibrosis or immuno-compromising conditions. Like other opportunistic Gram-negative bacteria, this pathogen establishes virulence and biofilms through lectin-mediated adhesion. In particular, the superlectin BC2L-C is believed to cross-link human epithelial cells to B. cenocepacia during pulmonary infections. We aimed to obtain glycomimetic antagonists able to inhibit the interaction between the N-terminal domain of BC2L-C (BC2L-C-Nt) and its target fucosylated human oligosaccharides. In a previous study, we identified by fragment virtual screening and validated a small set of molecular fragments that bind BC2L-C-Nt in the vicinity of the fucose binding site. Here, we report the rational design and synthesis of bifunctional C- or N-fucosides, generated by connecting these fragments to a fucoside core using a panel of rationally selected linkers. A modular route starting from two key fucoside intermediates was implemented for the synthesis, followed by evaluation of the new compounds as BC2L-C-Nt ligands with a range of techniques (surface plasmon resonance, isothermal titration calorimetry, saturation transfer difference NMR, differential scanning calorimetry, and X-ray crystallography). This study resulted in a hit molecule with an order of magnitude gain over the starting methyl fucoside and in two crystal structures of antagonist/lectin complexes.
Tipologia IRIS:
01 - Articolo su periodico
Elenco autori:
R. Bermeo, K. Lal, D. Ruggeri, D. Lanaro, S. Mazzotta, F. Vasile, A. Imberty, L. Belvisi, A. Varrot, A. Bernardi
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