Horizontal transmission of hypoviruses between vegetative compatibility types of Cryphonectria parasitica in Macedonia
Articolo
Data di Pubblicazione:
2008
Citazione:
Horizontal transmission of hypoviruses between vegetative compatibility types of Cryphonectria parasitica in Macedonia / I. Papazova-Anakieva, K. Sotirovski, P. Cortesi, M.G. Milgroom. - In: EUROPEAN JOURNAL OF PLANT PATHOLOGY. - ISSN 0929-1873. - 120:1(2008), pp. 35-42.
Abstract:
Biological control of chestnut blight with
hypovirulence depends on the successful transmission of hypoviruses between individuals of the chestnut blight fungus, Cryphonectria parasitica. Vegetative incompatibility inhibits horizontal virus transmission, but not completely. In an effort to assess the potential for the spread of hypoviruses in the Republic of Macedonia, we studied the transmission of Cryphonectria hypovirus 1 (CHV-1) among the five observed vegetative compatibility (vc) types of C. parasitica. One fungal isolate of each vc type was infected with CHV-1 and was paired in vitro with isolates of all other vc types for a total of 20 combinations of virus donors and recipients, and 250 replicate trials per combination. Virus transmission was scored after 7 days as successful if the recipient isolate took on an unpigmented culture phenotype typical of virus infection. Transmission occurred at high frequencies between some pairs of vc types, but in <1% of the
trials for 10 of the 20 combinations of donors and
recipients. Asymmetric transmission was observed
between some vc types that had different alleles at
vegetative incompatibility loci vic1 or vic7; i.e.,
transmission occurred at high frequencies in one
direction, but very low frequencies between the same pair of isolates in the opposite direction. The expected virus transmission, calculated as the average transmission predicted for any two randomly chosen individuals from a population, was highly negatively correlated to vc type diversity. Results for isolates of C. parasitica from Macedonia were similar to those from Italy, but less transmission was generally observed. Differences in genetic background effects
on transmission may vary among different populations even when isolates differ by the same vic alleles.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Biological control; Chestnut blight fungus; Hypovirulence; Mycovirus; Vegetative incompatibility; Virus transmission
Elenco autori:
I. Papazova-Anakieva, K. Sotirovski, P. Cortesi, M.G. Milgroom
Link alla scheda completa: