Sterols and sphingolipids as new players in cell wall building and apical growth of Nicotiana tabacum L. pollen tubes
Articolo
Data di Pubblicazione:
2023
Citazione:
Sterols and sphingolipids as new players in cell wall building and apical growth of Nicotiana tabacum L. pollen tubes / N. Stroppa, E. Onelli, P. Moreau, L. Maneta-Peyret, V. Berno, E. Cammarota, R. Ambrosini, M.S. Caccianiga, M. Scali, A. Moscatelli. - In: PLANTS. - ISSN 2223-7747. - 12:1(2023 Jan), pp. 8.1-8.31. [10.3390/plants12010008]
Abstract:
Pollen tubes are tip-growing cells that create safe routes to convey sperm cells to the
embryo sac for double fertilization. Recent studies have purified and biochemically characterized
detergent-insoluble membranes from tobacco pollen tubes. These microdomains, called lipid rafts,
are rich in sterols and sphingolipids and are involved in cell polarization in organisms
evolutionarily distant, such as fungi and mammals. The presence of actin in tobacco pollen tube
detergent-insoluble membranes and the preferential distribution of these domains on the apical
plasma membrane encouraged us to formulate the intriguing hypothesis that sterols and
sphingolipids could be a “trait d’union” between actin dynamics and polarized secretion at the tip.
To unravel the role of sterols and sphingolipids in tobacco pollen tube growth, we used squalestatin
and myriocin, inhibitors of sterol and sphingolipid biosynthesis, respectively, to determine whether
lipid modifications affect actin fringe morphology and dynamics, leading to changes in clear zone
organization and cell wall deposition, thus suggesting a role played by these lipids in successful
fertilization.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
pollen tube; apical growth; Nicotiana tabacum; lipid nanodomains; cell wall; vesicle trafficking; actin filaments; clear zone
Elenco autori:
N. Stroppa, E. Onelli, P. Moreau, L. Maneta-Peyret, V. Berno, E. Cammarota, R. Ambrosini, M.S. Caccianiga, M. Scali, A. Moscatelli
Link alla scheda completa: