The arabidopsis atfh frataxin : genetic approach with inducible rnai to investigate its role in the iron trafficking in mitochondria
Poster
Data di Pubblicazione:
2008
Citazione:
The arabidopsis atfh frataxin : genetic approach with inducible rnai to investigate its role in the iron trafficking in mitochondria / V. Vazzola, D. Tarantino, C. Soave, I. Murgia - In: Proceedings XLVII Congresso Società Italiana di Fisiologia Vegetale[s.l] : null, 2008. - pp. 58-58 (( Intervento presentato al 47. convegno Congresso Società Italiana Fisiologia Vegetale (SIFV) tenutosi a 2008 nel Pisa.
Abstract:
Frataxin is present in mitochondria of all eukaryotes as well as in the cytoplasm of bacteria.
Experimental evidence suggests that frataxin acts as an iron-chaperone protein, donating iron to the
proteins involved in [Fe-S] cluster assembly and heme synthesis. It also possibly contributes to the
process of iron detoxification and storage. The frataxin homolog from Arabidopsis thaliana (AtFH) is a
single nuclear-encoded gene targeted to mitochondria. We have previously shown that [1] the knocking
out of AtFH gene causes arrest of Arabidopsis embryo development at the globular stage. Consistently
with that, we have also shown by in situ hybridisation that AtFH is expressed, in wt Arabidopsis plants,
in ovule primordia as well as in embryos at various stages of development. We are currently producing
Arabidopsis transgenic lines, by inducible RNAinterference, in which microRNA can switch off AtFH
frataxin transcript accumulation upon plant treatment with either ethanol or dexametasone. Such
approach will allow to overcome experimental constraints due to embryo lethality of AtFH KO. [1]
Vazzola et al. (2007) FEBS Letters 581:667-672.
EXPRESSION
Tipologia IRIS:
03 - Contributo in volume
Keywords:
Frataxin ; iron ; mitochondria ; RNA interference
Elenco autori:
V. Vazzola, D. Tarantino, C. Soave, I. Murgia
Link alla scheda completa:
Titolo del libro:
Proceedings XLVII Congresso Società Italiana di Fisiologia Vegetale