Metabolomic and Proteomic Changes in Candida albicans Biofilm in Response to Zosteric Acid Treatment
Articolo
Data di Pubblicazione:
2022
Citazione:
Metabolomic and Proteomic Changes in Candida albicans Biofilm in Response to Zosteric Acid Treatment / C. Catto', L. Corte, L. Roscini, G. Cardinali, F. Villa, F. Cappitelli. - In: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES. - ISSN 1422-0067. - 23:22(2022 Nov 15), pp. 14067.1-14067.15. [10.3390/ijms232214067]
Abstract:
Zosteric acid (ZA) is a secondary metabolite of the seagrass Zostera marina, with antibiofilm
activity against fungi. Information concerning its mechanisms of action is lacking and this limits the
development of more potent derivatives based on the same target and activity structure. The aim
of this work was to investigate the ZA mode of action by analyzing the metabolic status of Candida
albicans biofilm and its protein expression profile upon ZA treatment. Fourier-Transform Infrared
Spectroscopy confirmed that ZA modified the metabolomic response of treated cells, showing changes
in the spectral regions, mainly related to the protein compartment. Nano Liquid Chromatography–
High-Resolution Mass Spectrometry highlighted that 10 proteins were differentially expressed in the
C. albicans proteome upon ZA treatment. Proteins involved in the biogenesis, structure and integrity
of cell walls as well as adhesion and stable attachment of hyphae were found downregulated, whereas
some proteins involved in the stress response were found overexpressed. Additionally, ZA was
involved in the modulation of non-DNA-based epigenetic regulatory mechanisms triggered by
reactive oxygen species. These results partially clarified the ZA mechanism of action against fungi
and provided insight into the major C. albicans pathways responsible for biofilm formation.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
zosteric acid; antibiofilm; natural compounds; mechanism of action; metabolomics; proteomics; Candida albicans
Elenco autori:
C. Catto', L. Corte, L. Roscini, G. Cardinali, F. Villa, F. Cappitelli
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