Comparative proteomic analysis of Lactobacillus plantarum WCFS1 and ΔctsR mutant strains under physiological and heat stress conditions
Articolo
Data di Pubblicazione:
2012
Citazione:
Comparative proteomic analysis of Lactobacillus plantarum WCFS1 and ΔctsR mutant strains under physiological and heat stress conditions / P. Russo, L.M. María, V. Capozzi, F.D.P. Pilar, L. Paloma, G. Spano, D. Fiocco. - In: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES. - ISSN 1422-0067. - 13:9(2012 Aug 24), pp. 10680-10696. [10.3390/ijms130910680]
Abstract:
Among Gram-positive bacteria, CtsR (Class Three Stress gene Repressor)
mainly regulates the expression of genes encoding the Clp ATPases and the ClpP protease.
To gain a better understanding of the biological significance of the CtsR regulon in
response to heat-shock conditions, we performed a global proteomic analysis of
Lactobacillus plantarum WCFS1 and ΔctsR mutant strains under optimal or heat stress
temperatures. Total protein extracts from bacterial cells were analyzed by two-dimensional
gel fractionation. By comparing maps from different culture conditions and different
L. plantarum strains, image analysis revealed 23 spots with altered levels of expression.
The proteomic analysis of L. plantarum WCFS1 and ctsR mutant strains confirms at the
translational level the CtsR-mediated regulation of some members of the Clp family, as
well as the heat induction of typical stress response genes. Heat activation of the putative
CtsR regulon genes at transcriptional and translational levels, in the ΔctsR mutant, suggests
additional regulative mechanisms, as is the case of hsp1. Furthermore, isoforms of ClpE
with different molecular mass were found, which might contribute to CtsR quality control.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
ClpE; CtsR; heat; Lactobacillus plantarum; stress
Elenco autori:
P. Russo, L.M. María, V. Capozzi, F.D.P. Pilar, L. Paloma, G. Spano, D. Fiocco
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