Reprogramming of dermal fibroblasts from a Duchenne muscular dystrophy patient carrying a deletion of exons 45–50 into an induced pluripotent stem cell line (CCMi005-A)
Articolo
Data di Pubblicazione:
2022
Citazione:
Reprogramming of dermal fibroblasts from a Duchenne muscular dystrophy patient carrying a deletion of exons 45–50 into an induced pluripotent stem cell line (CCMi005-A) / D. Rovina, E. Castiglioni, S. Mallia, M. Rabino, A. Farini, M. Belicchi, G. Di Giuseppe, C.C.G. Gervasini, Y. Torrente, G. Pompilio, A. Gowran. - In: STEM CELL RESEARCH. - ISSN 1876-7753. - 64:(2022 Oct), pp. 102889.1-102889.5. [10.1016/j.scr.2022.102889]
Abstract:
Duchenne muscular dystrophy (DMD) is an X-linked syndrome that affects skeletal and cardiac muscle and is caused by mutation of the dystrophin gene. Induced pluripotent stem cells (iPSCs) were generated from dermal fibroblasts by electroporation with episomal vectors containing the reprogramming factors (OCT4, SOX2, LIN28, KLF4, and L-MYC). The donor carried an out-of-frame deletion of exons 45-50 of the dystrophin gene. The established iPSC line exhibited normal morphology, expressed pluripotency markers, had normal karyotype and possessed trilineage differentiation potential.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Humans; Dystrophin; Inducible T-Cell Co-Stimulator Protein; Exons; Cell Differentiation; Fibroblasts; Cellular Reprogramming; Muscular Dystrophy, Duchenne; Induced Pluripotent Stem Cells
Elenco autori:
D. Rovina, E. Castiglioni, S. Mallia, M. Rabino, A. Farini, M. Belicchi, G. Di Giuseppe, C.C.G. Gervasini, Y. Torrente, G. Pompilio, A. Gowran
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