Data di Pubblicazione:
2006
Citazione:
Expression of phage P4 integrase is negatively regulated by both Int and Vis / D. Piazzolla, S. Calì, E. Spoldi, F. Forti, C. Sala, F. Magnoni, G. Dehò, D.E. Ghisotti. - In: JOURNAL OF GENERAL VIROLOGY. - ISSN 0022-1317. - 87:8(2006), pp. 2423-2431. [10.1099/vir.0.81875-0]
Abstract:
Phage P4 int gene encodes the integrase responsible for phage integration into and excision from the Escherichia coli chromosome. Here, the data showing that P4 int expression is regulated in a complex manner at different levels are presented. First of all, the Pint promoter is regulated negatively by both Int and Vis, the P4 excisionase. The N-terminal portion of Int appears to be sufficient for such a negative autoregulation, suggesting that the Int N terminus is implicated in DNA
binding. Second, full-length transcripts covering the entire int gene could be detected only upon P4 infection, whereas in P4 lysogens only short 59-end covering transcripts were detectable. On the other hand, transcripts covering the 59-end of int were also very abundant upon infection. It thus appears that premature transcription termination and/or mRNA degradation play a role in Int-negative regulation both on the basal prophage transcription and upon infection. Finally,comparison between Pint–lacZ transcriptional and translational fusions suggests that Vis regulates Int expression post-transcriptionally. The findings that Vis is also an RNA-binding protein and that Int may be translated from two different start codons have implications on possible regulation models of Int expression.
Tipologia IRIS:
01 - Articolo su periodico
Elenco autori:
D. Piazzolla, S. Calì, E. Spoldi, F. Forti, C. Sala, F. Magnoni, G. Dehò, D.E. Ghisotti
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