Data di Pubblicazione:
2008
Citazione:
The Impact of the Absence of Aliphatic Glucosinolates on
Insect Herbivory in Arabidopsis / J. Beekwilder, W. van Leeuwen, N.M. van Dam, M. Bertossi, V. Grandi, L. Mizzi, M. Soloviev, L. Szabados, J.W. Molthoff, B. Schipper, H. Verbocht, R.C.H. de Vos, P. Morandini, M.G.M. Aarts, A. Bovy. - In: PLOS ONE. - ISSN 1932-6203. - 3:4(2008 Apr), p. e2068.e2068. [10.1371/journal.pone.0002068]
Abstract:
Aliphatic glucosinolates are compounds which occur in high concentrations in Arabidopsis thaliana and other Brassicaceae
species. They are important for the resistance of the plant to pest insects. Previously, the biosynthesis of these compounds
was shown to be regulated by transcription factors MYB28 and MYB29. We now show that MYB28 and MYB29 are partially
redundant, but in the absence of both, the synthesis of all aliphatic glucosinolates is blocked. Untargeted and targeted
biochemical analyses of leaf metabolites showed that differences between single and double knock-out mutants and wild
type plants were restricted to glucosinolates. Biosynthesis of long-chain aliphatic glucosinolates was blocked by the myb28
mutation, while short-chain aliphatic glucosinolates were reduced by about 50% in both the myb28 and the myb29 single
mutants. Most remarkably, all aliphatic glucosinolates were completely absent in the double mutant. Expression of
glucosinolate biosynthetic genes was slightly but significantly reduced by the single myb mutations, while the double
mutation resulted in a drastic decrease in expression of these genes. Since the myb28myb29 double mutant is the first
Arabidopsis genotype without any aliphatic glucosinolates, we used it to establish the relevance of aliphatic glucosinolate
biosynthesis to herbivory by larvae of the lepidopteran insect Mamestra brassicae. Plant damage correlated inversely to the
levels of aliphatic glucosinolates observed in those plants: Larval weight gain was 2.6 fold higher on the double
myb28myb29 mutant completely lacking aliphatic glucosinolates and 1.8 higher on the single mutants with intermediate
levels of aliphatic glucosinolates compared to wild type plants.
Tipologia IRIS:
01 - Articolo su periodico
Elenco autori:
J. Beekwilder, W. van Leeuwen, N.M. van Dam, M. Bertossi, V. Grandi, L. Mizzi, M. Soloviev, L. Szabados, J.W. Molthoff, B. Schipper, H. Verbocht, R.C.H. de Vos, P. Morandini, M.G.M. Aarts, A. Bovy
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