Homocysteine measurement by an enzymatic method and potential role of homocysteine as a biomarker in dogs
Articolo
Data di Pubblicazione:
2008
Citazione:
Homocysteine measurement by an enzymatic method and potential role of homocysteine as a biomarker in dogs / S. Rossi, G. Rossi, A. Giordano, S. Paltrinieri. - In: JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION. - ISSN 1040-6387. - 20:5(2008), pp. 644-649.
Abstract:
In humans, homocysteine (Hcy) is employed to monitor renal, cardiovascular, and other
diseases and their complications. The aim of the current study was to define the analytical performances of an
enzymatic method not yet validated in dogs for measuring homocysteine, and to assess the possible clinical
usefulness of Hcy measurement. Using conventional approaches, the analytical performances were
investigated by assessing, imprecision, inaccuracy, and interference of hemoglobin, triglycerides, and
bilirubin. The possible clinical usefulness of Hcy determination was assessed by comparing the results of
healthy dogs (n 5 8) with those of dogs with heart disease (n 5 10), inflammation (n 5 6), gastrointestinal
disorders (n 5 7), neoplasia (n 5 8), renal failure (n 5 4), trauma (n 5 7), and other miscellaneous diseases (n
5 6). Preliminary evaluation of this enzymatic method showed it to be precise at Hcy levels close to or higher
than the values in dogs with renal or cardiac disorders that had the highest Hcy levels. By contrast, at low Hcy
levels, which were recorded basically in control dogs, the method suffers from high imprecision. The sample of
choice is serum. The use of icteric samples should be avoided, while hemoglobin and lipids have only a minor
effect on Hcy measurement. In conclusion, the enzymatic method employed in the current study provides
useful information in dogs and could be used to monitor cardiac and renal disorders, in which Hcy
concentrations are elevated.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Biomarker; Dogs; Enzymatic method; Heart disease; Homocysteine; Renal failure
Elenco autori:
S. Rossi, G. Rossi, A. Giordano, S. Paltrinieri
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