Structure-function studies on the complex iron-sulfur flavoprotein glutamate synthase: the key enzyme of ammonia assimilation.
Articolo
Data di Pubblicazione:
2005
Citazione:
Structure-function studies on the complex iron-sulfur flavoprotein glutamate synthase: the key enzyme of ammonia assimilation. / M.A. Vanoni, L. Dossena, R..H..H. van den Heuvel, B. Curti. - In: PHOTOSYNTHESIS RESEARCH. - ISSN 0166-8595. - 83:2(2005 Jan), pp. 219-238.
Abstract:
Glutamate synthases are complex iron sulfur flavoproteins that participate in the essential ammonia assimilation pathway in microorganisms and plants. The recent determination of the 3-dimensional structures of the alpha subunit of the NADPH-dependent glutamate synthase form and of the ferredoxin-dependent enzyme of Synechocystis sp. PCC 6803 provides a framework for the interpretation of the functional properties of these enzymes, and highlights protein segments most likely involved in control and coordination of the partial catalytic activities of glutamate synthases, which take place at sites distant from each other in space. In this review, we focus on the current knowledge on structure-function relationships in glutamate synthases, and we discuss open questions on the mechanisms of control of the enzyme reaction and of electron transfer among the enzyme flavin cofactors and iron-sulfur clusters.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
allosteric regulation ; amidotransferase ; ammonia assimilation ; electron transfer ; ferredoxin ; flavoprotein ; glutamate synthase ; iron-sulfur clusters ; oxidoreductases
Elenco autori:
M.A. Vanoni, L. Dossena, R..H..H. van den Heuvel, B. Curti
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