Effects of protein and peptides obtained from Okara, a by-product of soy-milk production, on human colon cancer Caco-2 cells
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Data di Pubblicazione:
2021
Citazione:
Effects of protein and peptides obtained from Okara, a by-product of soy-milk production, on human colon cancer Caco-2 cells / S. De Benedetti, G. Heinzl, C. Magni, S. Borgonovi, A. Scarafoni. ((Intervento presentato al 61. convegno SIB Congress tenutosi a Virtual edition: 23-24 September nel 2021.
Abstract:
Beyond the nutritive role, recent research pointed out the potential beneficial effects on human health
of foods proteins and peptides. These, may exert bioactivities, such as antioxidant and immunomodulating,
relevant for maintaining well-being and to prevent diseases. Soybean seed proteins have been extensively
reported in this respect.
Okara is a prime agri-waste generated by soybean milk processing industry that still contains high
amounts of nutrients, including proteins (25.4-28.4%) and polyphenols (up to 40 %) [1], [2] .
The aim of this study was to investigate the possible effects on inflammation and anti-proliferative
mechanisms of okara proteins (OP) and derived peptides, by using intestinal tumoral Caco-2 cells.
OP were obtained by selective solubilization, separated from the insoluble fiber fraction by
centrifugation, and concentrated by isoelectric precipitation at pH 4.5. Either the resulting supernatant (SN)
and precipitate (PP) were used for subsequent analyses. Okara peptides were generated by proteolysis with
pancreatin (OPEP1), and with pepsin followed by pancreatin (OPEP2), to simulate the gastro-intestinal
digestion. Protein and peptide fractions were characterized by using electrophoretic and chromatographic
methodologies.
The anti-proliferative potential was investigated by MTT assay, after incubation of Caco-2 cells with
okara samples. IL-8 gene expression was also assessed to evaluate the anti-inflammatory capacity of okara
products. Its expression, downstream the activation of NF-kB, the major regulator of cellular inflammatory
status was considered as a marker of inflammation [3] .
The results indicated that PP, OPEP1 and OPEP2 can cause cell suffering and death in a dose
dependent relation, whereas SN seems to exert no effects. No IL8 expression was observed in all cases.
Interestingly, treatment with okara samples strongly enhanced the expression of IL8 on cells elicited by IL1,
suggesting a synergic effect.
By and large, the observed cytotoxic effects may be due to the presence of peptides already present
in okara that may originate during the production of soybean drink.
[1] S. P. Stanojevic, M. B. Barac, M. B. Pesic, V. S. Jankovic, e B. V. Vucelic-Radovic, «Bioactive proteins
and energy value of okara as a byproduct in hydrothermal processing of soy milk», J. Agric. Food
Chem., vol. 61, n. 38, pagg. 9210–9219, set. 2013, doi: 10.1021/jf4012196.
[2] A. Orts et al., «Protease technology for obtaining a soy pulp extract enriched in bioactive compounds:
isoflavones and peptides», Heliyon, vol. 5, n. 6, pag. e01958, giu. 2019, doi:
10.1016/j.heliyon.2019.e01958.
[3] J. Capraro et al., «Characterization of Chenopodin Isoforms from Quinoa Seeds and Assessment of
Their Potential Anti-Inflammatory Activity in Caco-2 Cells», Biomolecules, vol. 10, n. 5, Art. n. 5, mag.
2020, doi: 10.3390/biom10050795.
Tipologia IRIS:
14 - Intervento a convegno non pubblicato
Elenco autori:
S. De Benedetti, G. Heinzl, C. Magni, S. Borgonovi, A. Scarafoni
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