Molecular response of Sargassum vulgare to acidification at volcanic CO2 vents: Insights from proteomic and metabolite analyses
Articolo
Data di Pubblicazione:
2022
Citazione:
Molecular response of Sargassum vulgare to acidification at volcanic CO2 vents: Insights from proteomic and metabolite analyses / A. Kumar, S. Nonnis, I. Castellano, H. Abdelgawad, G.T.S. Beemster, M. Cristina Buia, E.M. Maffioli, G. Tedeschi, A. Palumbo. - In: MOLECULAR ECOLOGY. - ISSN 1365-294X. - (2022). [Epub ahead of print] [10.1111/mec.16553]
Abstract:
Ocean acidification is impacting marine life all over the world. Understanding how
species can cope with the changes in seawater carbonate chemistry represents a
challenging issue. We addressed this topic using underwater CO2 vents that naturally
acidify some marine areas off the island of Ischia. In the most acidified area of the
vents, having a mean pH value of 6.7, comparable to far-future predicted acidification
scenarios (by 2300), the biomass is dominated by the brown alga Sargassum vulgare.
The novelty of the present study is the characterization of the S. vulgare proteome
together with metabolite analyses to identify the key proteins, metabolites, and path-
ways affected by ocean acidification. A total of 367 and 387 proteins were identified
in populations grown at pH that approximates the current global average (8.1) and
acidified sites, respectively. Analysis of their relative abundance revealed that 304
proteins are present in samples from both sites: 111 proteins are either higher or
exclusively present under acidified conditions, whereas 120 proteins are either lower
or present only under control conditions. Functionally, under acidification, a decrease
in proteins related to translation and post-translational processes and an increase of
proteins involved in photosynthesis, glycolysis, oxidation–reduction processes, and
protein folding were observed. In addition, small-molecule metabolism was affected,
leading to a decrease of some fatty acids and antioxidant compounds under acidifica-
tion. Overall, the results obtained by proteins and metabolites analyses, integrated with previous transcriptomic, physiological, and biochemical studies, allowed us to
delineate the molecular strategies adopted by S. vulgare to grow in future acidified
environments, including an increase of proteins involved in energetic metabolism,
oxidation–reduction processes, and protein folding at the expense of proteins involved in translation and post-translational processes.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
adaptation; CO2 seeps; macroalgae; metabolites; ocean acidification; proteins
Elenco autori:
A. Kumar, S. Nonnis, I. Castellano, H. Abdelgawad, G.T.S. Beemster, M. Cristina Buia, E.M. Maffioli, G. Tedeschi, A. Palumbo
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