Optimization of sperm cryopreservation protocol for mediterranean brown trout: A comparative study of non-permeating cryoprotectants and thawing rates in vitro and in vivo
Articolo
Data di Pubblicazione:
2019
Citazione:
Optimization of sperm cryopreservation protocol for mediterranean brown trout: A comparative study of non-permeating cryoprotectants and thawing rates in vitro and in vivo / G. Rusco, M.D. Iorio, P.P. Gibertoni, S. Esposito, M. Penserini, A. Roncarati, S. Cerolini, N. Iaffaldano. - In: ANIMALS. - ISSN 2076-2615. - 9:6(2019), pp. 304.1-304.12. [10.3390/ani9060304]
Abstract:
The aim of our study was to test the effects of different non-permeating cryoprotectants (NP-CPAs), namely low-density lipoproteins (LDLs), sucrose, and egg yolk, and thawing rates on the post-thaw semen quality and fertilizing ability of the native Mediterranean brown trout. Pooled semen samples were diluted 1:3 (v:v) with 2.5%, 5%, 10%, or 15% LDL; 0.05, 0.1, or 0.3 M sucrose; or 10% egg yolk. At the moment of analysis, semen was thawed at 30 °C/10 s or 10 °C/30 s. The post-thaw semen quality was evaluated, considering motility, the duration of motility, viability, and DNA integrity. Significantly higher values of motility and viability were obtained using egg yolk/10 °C for 30 s, across all treatments. However, LDL and sucrose concentrations affected sperm cryosurvival, showing the highest post-thaw sperm quality at 5% LDL and 0.1 M sucrose. Based on the in vitro data, egg yolk, 5% LDL, and 0.1 M sucrose thawed at 10 °C or 30 °C were tested for the in vivo trial. The highest fertilization and hatching rates were recorded using egg yolk/10 °C (p < 0.05). According to these in vitro and in vivo results, egg yolk emerged as the most suitable NP-CPA and 10 °C/30 s as the best thawing rate for the cryopreservation of this trout sperm, under our experimental conditions. View Full-Text
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Conservation biology; Egg yolk; Fertilization rate; Salmo cettii; Sperm cryobank; Sperm freezing
Elenco autori:
G. Rusco, M.D. Iorio, P.P. Gibertoni, S. Esposito, M. Penserini, A. Roncarati, S. Cerolini, N. Iaffaldano
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