Concentration dependent effect of dimethylacetamide and N-methylacetamide on the quality and fertility of cryopreserved chicken semen,
Articolo
Data di Pubblicazione:
2022
Citazione:
Concentration dependent effect of dimethylacetamide and N-methylacetamide on the quality and fertility of cryopreserved chicken semen, / L. Zaniboni, M. Madeddu, F. Mosca, A.A. Sayed, S.P. Marelli, M.D. Iorio, N. Iaffaldano, S. Cerolini. - In: CRYOBIOLOGY. - ISSN 0011-2240. - 106:(2022 Jun), pp. 66-72. [10.1016/j.cryobiol.2022.04.001]
Abstract:
The aim of this study was to compare the effect of two permeant-cryoprotectants, dimethylacetamide (DMA) and N-methylacetamide (NMA) used at different concentrations (0%, 2%, 4%, 6%) on the quality and fertility of post-thaw rooster semen. Ejaculates were processed in 7 treatments: Lake pre-freezing+0.1 M trehalose (LPF-T) (control treatment), LPF-T+2% DMA, LPF-T+4% DMA, LPF-T+6% DMA, LPF-T+2% NMA, LPF-T+4% NMA, LPF-T+6% NMA. Sperm quality [sperm membrane integrity (SMI), motility and kinetic parameters] was assessed before and after cryopreservation. Fertility and embryo viability were recorded. Increasing both DMA and NMA concentration from 2 to 6% improved SMI, total motile sperm, progressive motile sperm (PMS), VCL, VSL and VAP values. PMS recovery rates were significantly the highest in 6% DMA, 4% NMA and 6% NMA treatments. Semen cryopreserved with DMA produced the best fertility and embryo viability at 6%; progressive lower values were recorded at lower concentrations, with no viable embryos at 2%. Semen cryopreserved with NMA showed the best fertility values at 2% and lower values were recorded at higher concentrations; live embryos were found in all NMA treatments. Finally, NMA and DMA showed a similar positive concentration dependent effect of the quality of cryopreserved semen. NMA, not DMA, provided the highest fertility and embryo viability values at the lowest 2%. Therefore, the use of NMA is recommended in order to reduce the cryoprotectant concentration, with a concomitant reduction in the risk of toxicity, providing at the same time the adequate cryoprotective action to obtain viable embryos after artificial insemination of cryopreserved chicken semen.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Chicken semen; Cryoprotectant; Dimethylacetamide; Embryo viability; Fertility; N-methylcetamide; Sperm cryopreservation;
Elenco autori:
L. Zaniboni, M. Madeddu, F. Mosca, A.A. Sayed, S.P. Marelli, M.D. Iorio, N. Iaffaldano, S. Cerolini
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