Multifunctional, CD44v6-targeted ORMOSIL nanoparticles enhance drugs toxicity in cancer cells
Articolo
Data di Pubblicazione:
2020
Citazione:
Multifunctional, CD44v6-targeted ORMOSIL nanoparticles enhance drugs toxicity in cancer cells / L. Morillas-Becerril, E. Peta, L. Gabrielli, V. Russo, E. Lubian, L. Nodari, M.G. Ferlin, P. Scrimin, G. Palu, L. Barzon, I. Castagliuolo, F. Mancin, M. Trevisan. - In: NANOMATERIALS. - ISSN 2079-4991. - 10:2(2020 Feb 10), pp. 298.1-298.21. [10.3390/nano10020298]
Abstract:
Drug-loaded, PEGylated, organic-modified silica (ORMOSIL) nanoparticles prepared by microemulsion condensation of vinyltriethoxysilane (VTES) were investigated as potential nanovectors for cancer therapy. To target cancer stem cells, anti-CD44v6 antibody and hyaluronic acid (HA) were conjugated to amine-functionalized PEGylated ORMOSIL nanoparticles through thiol-maleimide and amide coupling chemistries, respectively. Specific binding and uptake of conjugated nanoparticles were studied on cells overexpressing the CD44v6 receptor. Cytotoxicity was subsequently evaluated in the same cells after the uptake of the nanoparticles. Internalization of nanocarriers loaded with the anticancer drug 3N-cyclopropylmethyl-7-phenyl-pyrroloquinolinone (MG2477) into cells resulted in a substantial increase of the cytotoxicity with respect to the free formulation. Targeting with anti-CD44v6 antibodies or HA yielded nanoparticles with similar effectiveness, in their optimized formulation.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Antibodies; CD44 receptor; Hyaluronic acid; Silica nanoparticles; Targeted delivery
Elenco autori:
L. Morillas-Becerril, E. Peta, L. Gabrielli, V. Russo, E. Lubian, L. Nodari, M.G. Ferlin, P. Scrimin, G. Palu, L. Barzon, I. Castagliuolo, F. Mancin, M. Trevisan
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