Data di Pubblicazione:
2022
Citazione:
Canine Spermatozoa—Predictability of Cryotolerance / S. Schäfer-Somi, M. Colombo, G.C. Luvoni. - In: ANIMALS. - ISSN 2076-2615. - 12:6(2022 Mar 15), pp. 733.1-733.12. [10.3390/ani12060733]
Abstract:
Markers of freezability allow the selection of ejaculates of good freezability. So far, most investigations
were conducted in boars, bulls, rams and horses, with high economic interests triggering
the efforts. The progress in dogs is comparably slow. A critical evaluation of the methods requires
consideration of practicability, with most labs not even possessing a computer assisted sperm analyser
(CASA); furthermore, small canine ejaculates mostly do not allow the use of large semen volumes.
In dogs, modern markers of freezability no longer assess single membrane constituents or seminal
plasma components but comprise tests of cell functionality and adaptability, energy metabolism,
cluster analyses of kinetic and morphometric parameters, as well as DNA intactness. Identification of
the most efficient combination of tests seems useful. At present, examination by CASA combined with
cluster analysis of kinetic subgroups, JC-1 staining and COMET assay or staining with toluidine blue
seem most appropriate; however, cell volumetry and other functional tests deserve better attention.
A better understanding of spermatozoa energy metabolism might reveal new markers. This review
focuses on the requirements and markers of freezability of canine semen, highlighting potential
future candidates.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
dogs; semen; cryopreservation; markers
Elenco autori:
S. Schäfer-Somi, M. Colombo, G.C. Luvoni
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