Data di Pubblicazione:
2022
Citazione:
Exercise and Training Regulation of Autophagy Markers in Human and Rat Skeletal Muscle / J. Botella, N.A. Jamnick, C. Granata, A.J. Genders, E. Perri, T. Jabar, A. Garnham, M. Lazarou, D.J. Bishop. - In: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES. - ISSN 1422-0067. - 23:5(2022 Feb 27), pp. 2619.1-2619.15. [10.3390/ijms23052619]
Abstract:
Autophagy is a key intracellular mechanism by which cells degrade old or dysfunctional
proteins and organelles. In skeletal muscle, evidence suggests that exercise increases autophagosome
content and autophagy flux. However, the exercise-induced response seems to differ between rodents
and humans, and little is known about how different exercise prescription parameters may affect these
results. The present study utilised skeletal muscle samples obtained from four different experimental
studies using rats and humans. Here, we show that, following exercise, in the soleus muscle of Wistar
rats, there is an increase in LC3B-I protein levels immediately after exercise (+109%), and a subsequent
increase in LC3B-II protein levels 3 h into the recovery (+97%), despite no change in Map1lc3b mRNA
levels. Conversely, in human skeletal muscle, there is an immediate exercise-induced decrease in
LC3B-II protein levels (−24%), independent of whether exercise is performed below or above the
maximal lactate steady state, which returns to baseline 3.5 h following recovery, while no change in
LC3B-I protein levels or MAP1LC3B mRNA levels is observed. SQSTM1/p62 protein and mRNA
levels did not change in either rats or humans following exercise. By employing an ex vivo autophagy
flux assay previously used in rodents we demonstrate that the exercise-induced decrease in LC3B-II
protein levels in humans does not reflect a decreased autophagy flux. Instead, effect size analyses
suggest a modest-to-large increase in autophagy flux following exercise that lasts up to 24 h. Our
findings suggest that exercise-induced changes in autophagosome content markers differ between
rodents and humans, and that exercise-induced decreases in LC3B-II protein levels do not reflect
autophagy flux level
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
autophagy; exercise; LC3; skeletal muscle;
Elenco autori:
J. Botella, N.A. Jamnick, C. Granata, A.J. Genders, E. Perri, T. Jabar, A. Garnham, M. Lazarou, D.J. Bishop
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