Astrocytes increase ATP exocytosis mediated calcium signaling in response to microgroove structures
Articolo
Data di Pubblicazione:
2015
Citazione:
Astrocytes increase ATP exocytosis mediated calcium signaling in response to microgroove structures / A.V. Singh, M. Raymond, F. Pace, A. Certo, J.M. Zuidema, C.A. Mckay, R.J. Gilbert, X.L. Lu, L.Q. Wan. - In: SCIENTIFIC REPORTS. - ISSN 2045-2322. - 5:1(2015), pp. 7847.1-7847.8. [10.1038/srep07847]
Abstract:
Following central nervous system (CNS) injury, activated astrocytes form glial scars, which inhibit axonal regeneration, leading to long-term functional deficits. Engineered nanoscale scaffolds guide cell growth and enhance regeneration within models of spinal cord injury. However, the effects of micro-/nanosize scaffolds on astrocyte function are not well characterized. In this study, a high throughput (HTP) microscale platform was developed to study astrocyte cell behavior on micropatterned surfaces containing 11/4m spacing grooves with a depth of 250 or 500â €...nm. Significant changes in cell and nuclear elongation and alignment on patterned surfaces were observed, compared to on flat surfaces. The cytoskeleton components (particularly actin filaments and focal adhesions) and nucleus-centrosome axis were aligned along the grooved direction as well. More interestingly, astrocytes on micropatterned surfaces showed enhanced mitochondrial activity with lysosomes localized at the lamellipodia of the cells, accompanied by enhanced adenosine triphosphate (ATP) release and calcium activities. These data indicate that the lysosome-mediated ATP exocytosis and calcium signaling may play an important role in astrocytic responses to substrate topology. These new findings have furthered our understanding of the biomechanical regulation of astrocyte cell-substrate interactions, and may benefit the optimization of scaffold design for CNS healing.
Tipologia IRIS:
01 - Articolo su periodico
Elenco autori:
A.V. Singh, M. Raymond, F. Pace, A. Certo, J.M. Zuidema, C.A. Mckay, R.J. Gilbert, X.L. Lu, L.Q. Wan
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