Enteric Polymer-Coated Porous Silicon Nanoparticles for Site-Specific Oral Delivery of IgA Antibody
Articolo
Data di Pubblicazione:
2020
Citazione:
Enteric Polymer-Coated Porous Silicon Nanoparticles for Site-Specific Oral Delivery of IgA Antibody / T. Kumeria, J. Wang, B. Kim, J.-. Park, J.M. Zuidema, M. Klempner, L. Cavacini, Y. Wang, M.J. Sailor. - In: ACS BIOMATERIALS SCIENCE & ENGINEERING. - ISSN 2373-9878. - (2020). [Epub ahead of print] [10.1021/acsbiomaterials.0c01313]
Abstract:
Porous silicon (pSi) nanoparticles are loaded with Immunoglobulin A-2 (IgA2) antibodies, and the assembly is coated with pH-responsive polymers on the basis of the Eudragit family of enteric polymers (L100, S100, and L30-D55). The temporal release of the protein from the nanocomposite formulations is quantified following an in vitro protocol simulating oral delivery: incubation in simulated gastric fluid (SGF; at pH 1.2) for 2 h, followed by a fasting state simulated intestinal fluid (FasSIF; at pH 6.8) or phosphate buffer solution (PBS; at pH 7.4). The nanocomposite formulations display a negligible release in SGF, while more than 50% of the loaded IgA2 is released in solutions at a pH of 6.8 (FasSIF) or 7.4 (PBS). Between 21 and 44% of the released IgA2 retains its functional activity. A capsule-based system is also evaluated, where the IgA2-loaded particles are packed into a gelatin capsule and the capsule is coated with either EudragitL100 or EudragitS100 polymer for a targeted release in the small intestine or the colon, respectively. The capsule-based formulations outperform polymer-coated nanoparticles in vitro, preserving 45-54% of the activity of the released protein.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
biologic antibacterial therapeutics, Eudragit polymer; oral drug delivery; pH-responsive drug delivery
Elenco autori:
T. Kumeria, J. Wang, B. Kim, J.-. Park, J.M. Zuidema, M. Klempner, L. Cavacini, Y. Wang, M.J. Sailor
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