Data di Pubblicazione:
2020
Citazione:
Analysis of cellular immuner in lepidopteran larvae / A. Becchimanzi, I. Di Lelio, F. Pennacchio, S. Caccia (SPRINGER PROTOCOLS HANDBOOKS). - In: Immunity in insects / [a cura di] F. Sandrelli, G. Tettamanti. - [s.l] : Humana, 2020. - ISBN 978-1-0716-0258-4. - pp. 97-111 [10.1007/978-1-0716-0259-1_6]
Abstract:
The immune system in insects is innate and relies both on humoral components and cellular response, which includes phagocytosis, nodulation, and encapsulation. Humoral and cellular defenses are inextricably linked in a complex immune reaction, modulated by a number of cross-talking pathways, which can result in a remarkable variability of any measurement. Protocols to assess the immune response are quite critical and should be carefully standardized, in order to achieve a desirable level of reproducibility. Here we report methods for measuring the cellular immune response in insects, using lepidopteran larvae as model. These protocols have been developed starting from the fragmented information available in the literature, which has been integrated and refined on the basis of our experience on the study of insect immunosuppression by parasitoids and pathogens.
Tipologia IRIS:
03 - Contributo in volume
Keywords:
Insect immunity; Hemocytes; Phagocytosis; Nodulation; Encapsulation
Elenco autori:
A. Becchimanzi, I. Di Lelio, F. Pennacchio, S. Caccia
Link alla scheda completa:
Titolo del libro:
Immunity in insects