Detection and monitoring of fungicide resistance in the rice blast pathogen by innovative molecular methods
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Data di Pubblicazione:
2021
Citazione:
Detection and monitoring of fungicide resistance in the rice blast pathogen by innovative molecular methods / A. Kunova, V. Tava, M. Pasquali, M. Saracchi, P. Cortesi. ((Intervento presentato al convegno APPS online conference: Australasian Plant Pathology Society Online Conference: Stay connected with plant pathology education tenutosi a [online] nel 2021.
Abstract:
Rice blast, caused by Pyricularia oryzae, is one of the most important rice diseases worldwide, causing globally 30% yield losses, threatening thus global food security. Rice blast management relies on fungicides, especially in Europe, as susceptible traditional rice varieties are extensively grown. Often, the first choice among a very limited spectrum of chemical control means are quinone outside inhibitor (QoI) fungicides. However, they are classified as high-risk fungicides as they are prone to resistance development in fungal pathogens. Until now, QoI resistance has been detected in ca. 40 different plant pathogens. Recently, azoxystrobin-resistant strains have been identified in P. oryzae from rice in Japan and Vietnam, which poses concerns about the spread of QoI resistance also in other rice-growing areas. Despite this, no reliable and sensitive detection method of QoI resistance in P. oryzae populations exists at the moment.
We developed an allele-specific qPCR-based method for the detection of QoI-resistant strains of P. oryzae. It is based on selective amplification of sensitive (S) or resistant (R) allele of cytochrome b. The method was developed using plasmids with cloned cytochrome b fragment containing or not the single-point mutation and was further verified on genomic DNA mixtures isolated from sensitive and resistant P. oryzae strains. Moreover, the specificity of primers was verified on several non-target fungal pathogens.
Tipologia IRIS:
14 - Intervento a convegno non pubblicato
Elenco autori:
A. Kunova, V. Tava, M. Pasquali, M. Saracchi, P. Cortesi
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