Fatty acid desaturase involvement in non-alcoholic fatty liver disease rat models : Oxidative stress versus metalloproteinases
Articolo
Data di Pubblicazione:
2019
Citazione:
Fatty acid desaturase involvement in non-alcoholic fatty liver disease rat models : Oxidative stress versus metalloproteinases / G. Palladini, L.G. Di Pasqua, C. Berardo, V. Siciliano, P. Richelmi, B. Mannucci, A.C. Croce, V. Rizzo, S. Perlini, M. Vairetti, A. Ferrigno. - In: NUTRIENTS. - ISSN 2072-6643. - 11:4(2019), pp. 799.1-799.14. [10.3390/nu11040799]
Abstract:
We investigated changes in fatty acid desaturases, D5D, D6D, D9-16D and D9-18D, and their relationship with oxidative stress, matrix metalloproteinases (MMPs) and serum TNF-alpha in two rat models of non-alcoholic fatty liver disease NAFLD. Eight-week-old male Wistar rats fed for 3 weeks with methionine-choline⁻deficient (MCD) diet and eleven-week-old Obese male Zucker rats were used. Serum levels of hepatic enzymes and TNF-alpha were quantified. Hepatic oxidative stress (ROS, TBARS and GSH content) and MMP-2 and MMP-9 (protein expression and activity) were evaluated. Liver fatty acid profiling, performed by GC-MS, was used for the quantification of desaturase activities. Higher D5D and D9-16D were found in Obese Zucker rats as well as an increase in D9-18D in MCD rats. D6D was found only in MCD rats. A negative correlation between D5D and D9-16D versus TBARS, ROS and TNF-alpha and a positive correlation with GSH were shown in fatty livers besides a positive correlation between D9-18D versus TBARS, ROS and TNF-alpha and a negative correlation with GSH. A positive correlation between D5D or D9-16D or D9-18D versus protein expression and the activity of MMP-2 were found. NAFLD animal models showed comparable serum enzymes. These results reinforce and extend findings on the identification of therapeutic targets able to counteract NAFLD disorder.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Desaturase; Fatty liver; Metalloproteinases; Oxidative stress; TNF-alpha; Animals; Disease Models; Animal; Fatty Acid Desaturases; Liver; Male; Matrix Metalloproteinase 2; Matrix Metalloproteinase 9; Non-alcoholic Fatty Liver Disease; Oxidative Stress; Rats; Rats; Wistar; Rats; Zucker
Elenco autori:
G. Palladini, L.G. Di Pasqua, C. Berardo, V. Siciliano, P. Richelmi, B. Mannucci, A.C. Croce, V. Rizzo, S. Perlini, M. Vairetti, A. Ferrigno
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