Data di Pubblicazione:
2007
Citazione:
miRNA DISCOVERY IN GRAPEVINE BY IN SILICO APPROACHES / E. Mica, V. Piccolo, M.E. Pè, G. Pesole, D. Horner - In: Proceedings of the 51st Italian Society of Agricultural Genetics Annual Congress[s.l] : null, 2007. - ISBN 978-88-900622-7-8. (( Intervento presentato al 51. convegno Italian Society of Agricultural Genetics Annual Congress tenutosi a Riva del Garda nel 2007.
Abstract:
The key role of non-coding RNAs in many different biological areas has become clear in the
last ten years. microRNAs are a group of non-coding small RNAs (20-22 nt long) which are often
conserved between related organisms and which are coded by specific genes called MIR genes. All
the transcripts that give rise to the same miRNA are grouped in a single family.
microRNAs are involved in post-transcriptional gene regulation, inducing transcript cleavage
or translation inhibition, throughout sequence similarity. Target genes are associated with many
different biological processes and possess no significant similarity to their respective miRNA genes,
apart from the 21 nucleotide sequence. In plants, target genes are typically involved in stress
response and plant development. They are broadly present in the plant kingdom; in recent years
many new species have been investigated and many miRNAs have been discovered, mainly through
in silico comparative genomic approaches.
Here we present a double in silico approach applied to the complete sequence of the grape
(Vitis vinifera L.) genome focused on the discovery of both conserved plant miRNAs and new
species-specific miRNAs. Interesting comparative and phylogenetic analyses have been performed
that explicit how different miRNA gene families behave during evolution, following geneexpansion
or gene-loss, showing in some cases a similar genomic organization, among different
species.
Moreover, to further characterize these miRNAs and investigate their putative roles, a detailed
target analysis has been performed. As expected targets of related families are highly conserved
between species, belonging to the same functional categories. Nonetheless a further characterization
of these targets and their corresponding miRNAs can clarify their role and possible involvement in
plant growth and fruit development.
Tipologia IRIS:
03 - Contributo in volume
Keywords:
microRNA ; grapevine ; genomics
Elenco autori:
E. Mica, V. Piccolo, M.E. Pè, G. Pesole, D. Horner
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Titolo del libro:
Proceedings of the 51st Italian Society of Agricultural Genetics Annual Congress