Structural insights into fusion mechanisms of small extracellular vesicles with model plasma membranes
Articolo
Data di Pubblicazione:
2021
Citazione:
Structural insights into fusion mechanisms of small extracellular vesicles with model plasma membranes / F. Perissinotto, V. Rondelli, B. Senigagliesi, P. Brocca, L. Almasy, L. Bottyan, D.G. Merkel, H. Amenitsch, B. Sartori, K. Pachler, M. Mayr, M. Gimona, E. Rohde, L. Casalis, P. Parisse. - In: NANOSCALE. - ISSN 2040-3364. - 13:10(2021), pp. 5224-5233. [10.1039/d0nr09075a]
Abstract:
Extracellular vesicles (EVs) are a potent intercellular communication system. Such small vesicles transport biomolecules between cells and throughout the body, strongly influencing the fate of recipient cells. Due to their specific biological functions they have been proposed as biomarkers for various diseases and as optimal candidates for therapeutic applications. Despite their extreme biological relevance, their mechanisms of interaction with the membranes of recipient cells are still hotly debated. Here, we propose a multiscale investigation based on atomic force microscopy, small angle X-ray scattering, small angle neutron scattering and neutron reflectometry to reveal structure-function correlations of purified EVs in interaction with model membrane systems of variable complex compositions and to spot the role of different membrane phases on the vesicle internalization routes. Our analysis reveals strong interactions of EVs with the model membranes and preferentially with the borders of protruding phase domains. Moreover, we found that upon vesicle breaking on the model membrane surface, the biomolecules carried by/on EVs diffuse with different kinetics rates, in a process distinct from simple fusion. The biophysical platform proposed here has clear implications on the modulation of EV internalization routes by targeting specific domains at the plasma cell membrane and, as a consequence, on EV-based therapies.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Cell Communication; Cell Membrane; Microscopy, Atomic Force; Extracellular Vesicles
Elenco autori:
F. Perissinotto, V. Rondelli, B. Senigagliesi, P. Brocca, L. Almasy, L. Bottyan, D.G. Merkel, H. Amenitsch, B. Sartori, K. Pachler, M. Mayr, M. Gimona, E. Rohde, L. Casalis, P. Parisse
Link alla scheda completa:
Link al Full Text: