Data di Pubblicazione:
2009
Citazione:
Prepubertal and adult ovine oocytes show different sensitivity to vitrification procedures / S. Succu, G.G. Leoni, M. Madeddu, V. Satta, L. Bogliolo, D. Bebbere, A. Spezzigu, A. Tedde, F. Berlinguer, S. Ledda, S. Naitana. - In: COMPTES RENDUS DE L'ACADÉMIE BULGARE DES SCIENCES. - ISSN 1310-1331. - 62:9(2009), pp. 1119-1124. ((Intervento presentato al 1. convegno Conference of the Balkan Network for Animal Reproduction Biotechnology : February, 18th - 19th tenutosi a Sofia nel 2009.
Abstract:
The aim of our work was to assess the effect of cryoprotectants and vitri- fication procedures on prepubertal ovine oocytes. Prepubertal and adult COCs were matured in vitro and exposed to cryoprotectants (EXP) or vitrified/warmed (VIT) using the cryoloop as cryodevice. After treatment, we evaluated in vitro the viability and development and chromatin alterations. Prepubertal and adult oocytes did not differ (p > 0.05) in degeneration and cleavage rates both in EXP and VIT groups. In the EXP group, prepubertal blastocyst output was lower (p < 0.05) than in the adults, while it did not differ in VIT group. Chromatin alterations were more evident in prepubertal than in adult oocytes both in EXP and VIT groups (p < 0.01). In conclusion, prepubertal ovine oocytes show a high sensitivity to vitrification procedures and their use for cryobanking require more studies.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Oocyte; Prepubertal; Vitrification
Elenco autori:
S. Succu, G.G. Leoni, M. Madeddu, V. Satta, L. Bogliolo, D. Bebbere, A. Spezzigu, A. Tedde, F. Berlinguer, S. Ledda, S. Naitana
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