Data di Pubblicazione:
2009
Citazione:
Cryotolerance and gene expression of in vitro produced prepubertal goat blastocysts / G. Leoni, S. Succu, V. Satta, L. Bogliolo, D. Bebbere, A. Spezzigu, M. Madeddu, A. Tedde, F. Berlinguer, S. Ledda, S. Naitana. - In: COMPTES RENDUS DE L'ACADÉMIE BULGARE DES SCIENCES. - ISSN 1310-1331. - 62:3(2009), pp. 367-372. (Intervento presentato al 1. convegno Conference of the Balkan-Network-for-Animal-Reproduction-Biotechnology : February, 18th - 19th tenutosi a Sofia nel 2009).
Abstract:
Twelve prepubertal (35-45-day-old) and 13 adult (3-6-year-old) goats were stimulated and COCs were collected by OPU and matured in vitro. After oocyte in vitro fertilization and culture, blastocysts were vitrified/warmed and cultured in vitro to assess blastocoelic re-expansion and gene expression. Prepubertals produced more COCs (p < 0.01) than adults. Degenerated COCs number (p < 0.01) was higher in prepubertals than adults and lower cleavage (p < 0.01) and blastocyst rates (p < 0.05) were evidenced in prepubertal compared to adult fertilized oocytes. Prepubertal vitrified/warmed blastocysts present a lower viability (p < 0.05) than adults. No significant differences were found in the expression of Cyclin B1, E-caderin, Na/K-ATPase, HSP90 and Aquaporin 3 genes. In conclusion our results evidenced a low development and cryotolerance of prepubertal goat embryos. However, the quality of viable prepubertal embryos after vitrification do not differ from the adult counterpart.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Cryotolerance; Gene expression; Prepubertal embryos
Elenco autori:
G. Leoni, S. Succu, V. Satta, L. Bogliolo, D. Bebbere, A. Spezzigu, M. Madeddu, A. Tedde, F. Berlinguer, S. Ledda, S. Naitana
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