Effects of a trichothecene, T-2 toxin, on proliferation and steroid production by porcine granulosa cells
Articolo
Data di Pubblicazione:
2009
Citazione:
Effects of a trichothecene, T-2 toxin, on proliferation and steroid production by porcine granulosa cells / F. Caloni, G. Ranzenigo, F. Cremonesi, L.J. Spicer. - In: TOXICON. - ISSN 0041-0101. - 54:3(2009 Sep), pp. 337-344.
Abstract:
Fusarium mycotoxins, such as trichothecenes and zearalenone, are produced by molds and
contaminate a large variety of grains and feedstuffs worldwide. Mycotoxins of Fusarium
fungi include the trichothecenes, deoxynivalenol and T-2 toxin (T2), and zearalenone, and
have been implicated in poor reproductive performance in pigs. However, direct ovarian
effects of T2 toxin have not been reported. Therefore, porcine granulosa cells (GC) from
small follicles (1–5 mm) were cultured for 2 days in 5% fetal bovine serum and 5% porcine
serum-containing medium followed by 2 days in serum-free medium containing various
doses of FSH, insulin-like growth factor-I and T2 (at various doses/combinations) to
evaluate the influence of T2 on steroid production and cell proliferation. T2 at 1, 3, 30 and
300 ng/mL completely inhibited FSH plus IGF-I-induced estradiol production, whereas
0.3 ng/mL of T2 inhibited estradiol production by 40%. Progesterone production was less
sensitive to the inhibitory effects of T2 with 0.3 ng/mL having no effect and 1 ng/mL
inhibiting progesterone production by only 30%. At 30 and 300 ng/mL, T2 completely
inhibited FSH plus IGF-I-induced progesterone production. The impact of T2 on the dose–
response to IGF-I (0, 3, 10 and 30 ng/mL) was also evaluated; T2 blunted the stimulatory
effect of 3–30 ng/mL of IGF-I on steroid production and cell proliferation. Serum-induced
granulosa cell proliferation was decreased (P < 0.05) by 40% after 1 day and by 56% after
2 days of T2 treatment. The present studies indicate for the first time that T2 may be able
to alter in growth of the granulosa layer within ovarian follicles in addition to their effect
on steroidogenesis. In conclusion, T2 has potent direct dose-dependent effects on granulosa
cell proliferation and steroidogenesis. These direct ovarian effects could be one
mechanism whereby contaminating Fusarium mycotoxins in feedstuffs could impact
reproductive performance in swine.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Trichothecenes ; T-2 toxin ; Granulosa cells ; Swine
Elenco autori:
F. Caloni, G. Ranzenigo, F. Cremonesi, L.J. Spicer
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