Immunocytochemical expression of human muscle cell p75 neurotrophin receptor is down-regulated by cyclic adenosine 3',5'-monophosphate
Articolo
Data di Pubblicazione:
1997
Citazione:
Immunocytochemical expression of human muscle cell p75 neurotrophin receptor is down-regulated by cyclic adenosine 3',5'-monophosphate / P. Baron, E. Scarpini, S. Pizzul, F. Zotti, G. Conti, D. Pleasure, G. Scarlato. - In: NEUROSCIENCE LETTERS. - ISSN 0304-3940. - 234:2-3(1997), pp. 79-82.
Abstract:
To investigate whether the immunocytochemical expression of low affinity neurotrophin receptor (p75) in human muscle is modulated by increased levels of intracellular cyclic adenosine 3',5'-monophoshate (cAMP), human cultured myogenic cells were treated with cAMP analogues dibutyryl cAMP (dbcAMP 0.5-1 mM) and 8-bromo cAMP (1 mM) or the adenylate cyclase activator forskolin (10- 100 μM). Cultures were processed for indirect immunofluorescence microscopy using an anti-human p75 mAb. The treatment of cultured muscle cells with cAMP analogues or forskolin for two days induced a decrease of immunoreactivity for p75 and a reduction of both myotube formation and morphological cell differentiation. Removal of cAMP derivatives from the medium resulted in a return of immunoreactive cells to the levels of untreated controls. These data indicate that adenylate cyclase is involved in the regulation of human muscle p75.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Adenosine 3',5'- monophosphate; Human muscle cells; Immunocytochemistry; p75
Elenco autori:
P. Baron, E. Scarpini, S. Pizzul, F. Zotti, G. Conti, D. Pleasure, G. Scarlato
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