Proinflammatory profile of cytokine production by human monocytes and murine microglia stimulated with beta-amyloid[25-35]
Articolo
Data di Pubblicazione:
1999
Citazione:
Proinflammatory profile of cytokine production by human monocytes and murine microglia stimulated with beta-amyloid[25-35] / L. Meda, P. Baron, E. Prat, E. Scarpini, G. Scarlato, M.A. Cassatella, F. Rossi. - In: JOURNAL OF NEUROIMMUNOLOGY. - ISSN 0165-5728. - 93:1-2(1999), pp. 45-52.
Abstract:
Growing evidence indicates that amyloid (A beta) deposition and phagocyte activation participate in inflammatory reactions in the brain during the course of Alzheimer's disease. To further investigate the effects of A beta-phagocyte interaction, we examined the production of proinflammatory (IL-1beta, IL-6), chemotactic (MIP-1alpha, IP-10) and inhibitory (IL-1Ra, IL-10 and TGFbeta1) cytokines by cultured human monocytes and mouse microglial cells upon stimulation with A beta[25-35]. Northern blot analysis and specific immunoassays demonstrated that A beta[25-35] triggers mRNA expression and release of IL-1beta, IL-1Ra and MIP-1alpha but not of IL-6, IL-10, TGFbeta1 and IP-10 from human monocytes. Similar results were obtained by examining the production of IL-1beta, IL-6 and IL-10 from mouse microglial cells in the same experimental conditions. Taken together, these data indicate that A beta-phagocyte interaction can drive a different response towards cytokine production by monocytes and microglia, with a particular proinflammatory trend, and further support a role for A beta deposition as a triggering factor of inflammatory events in Alzheimer's disease.
Tipologia IRIS:
01 - Articolo su periodico
Elenco autori:
L. Meda, P. Baron, E. Prat, E. Scarpini, G. Scarlato, M.A. Cassatella, F. Rossi
Link alla scheda completa: