Whole blood cytokine profiles in cats infected by feline coronavirus and healthy non-FCoV infected specific pathogen free cats
Articolo
Data di Pubblicazione:
2006
Citazione:
Whole blood cytokine profiles in cats infected by feline coronavirus and healthy non-FCoV infected specific pathogen free cats / M.E. Gelain, M. Meli, S. Paltrinieri. - In: JOURNAL OF FELINE MEDICINE AND SURGERY. - ISSN 1098-612X. - 8:6(2006), pp. 389-399.
Abstract:
In this study, the cytokine profiles of clinically healthy cats naturally infected with feline coronavirus (FCoV), of cats with feline infectious peritonitis (FIP) and of specific pathogen-free (SPF) cats were investigated in whole blood using a traditional reverse-transcriptase polymerase chain reaction (RT-PCR) assay and a semi-quantitative method of analysis based on computerised quantification of positive bands. The low inter-assay coefficient of variation recorded demonstrated that this method is highly repeatable. Compared with SPF cats, cytokine production was upregulated in most of the samples from FCoV-positive non-symptomatic cats. The appearance of a case of FIP in the cattery was associated with an increased expression of cytokines, in particular there was an increased production of IL-1β and IFN-γ, suggesting that these cytokines might protect infected cats from the disease. This hypothesis was also supported by the low levels of IFN-γ recorded in blood from cats with FIP.
Tipologia IRIS:
01 - Articolo su periodico
Elenco autori:
M.E. Gelain, M. Meli, S. Paltrinieri
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